![]() Lassa virus, nucleoprotein, arena viruses, The nucleotide diversity was also very high using the Taijima’s model in MEGA 4.ÂĬonclusion: The divergence within strains always coincides with the period of epidemic which goes to confirm that the cause of epidemic outbreak should be the emergence of new strain. These figures are far higher than an earlier study using Lassa virus glycoprotein. The overall transition/transversion bias R=16.662 with a total of 620 position in the final data set. The transition/transversion ratio k1 = 14.991 (purines) and k2 = 69.916 (pyrimidines). In synonymous substitution the rate of (G-T) rare was high. However the first human appearance of the virus was predicted to be around May 1959. The time of emergence of Lassa virus was predicted to be around January 1920. The phylogenetic tree was inferred by unweighted pairwise grouping in MEGA4 and using neighbour-joining method. The average number of nonsynonymous sites is 392.259. The average number of synonymous sites is 150.741. The average predicted rate of synonymous and nonsynonymous using modified Nei-Gojobori (assuming transition/transversion bias = 2) was 27.9 which was taken as the genetic distance between strains. The rate of synonymous substitution was high 5.889 per nucleotide per year and nonsynonymous was higher at 49.664. The genetic distances among strains were predicted by pairwise nucleotide differences. Methods and Findings: The nucleotide sequences of 18 Lassa virus genomic RNA encoding Lassa virus nucleoprotein isolates collected from different parts of the world were obtained from the GenBank and nucleotide substitution among them studied using the computer program MEGA4. Molecular evolution was studied through virulence diversity through to provide insight as to despite circulating antibodies there is still yearly epidemic outbreaks. Background: Lassa virus is the cause of Lassa fever with high morbidity and mortality.
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